Amino Acid Analysis of Liver Biopsy Sample

Fourteen amino acids in a liver biopsy sample were separated using a GROM-SIL OPA-1 column after precolumn derivatization with OPA and detected by fluorescence spectroscopy at 330 and 450nm.

Amino Acid Analysis of Liver Biopsy Sample Using OPA for Precolumn Derivatisation, Grom Application Number 10061

Fourteen amino acids in a liver biopsy sample were separated using a GROM-SIL OPA-1 column (125 x 4mm, Part No. GSOP10308S1204) after precolumn derivatization with OPA by gradient elution with 12.5mM sodium phosphate, pH 7.2 and acetonitrile 97/2 and 12.5mM sodium phosphate, pH 7.2 and acetonitrile 50/50 in around 14 minutes and detected by fluorescence spectroscopy at 330 and 450nm. The amino acids separated were glutamic acid, alpha-aminoadipic acid, serine, glutamine, glycine, histidine, citrulline, 3-methylhistidine, arginine, tyrosine, valine, nor-valine, isoleucine, phenylalanine, leucine and lysine.