Application Notes
Non-Ionic Detergent Glucopyranosides by HPLC/ELSD, UV
Glucopyranosides in non-ionic detergent were separated by reversed phase HPLC and detected by evaporative light scattering and UV at 220nm.
Non-Ionic Detergent Glucopyranosides, Alltech Application Note 0010E, May 2, 2000.
Glucopyranosides in non-ionic detergent were separated isocratically in around 5 minutes by reversed phase HPLC on an Econosphere C18, 5µm, 150 x 4.6mm column (Part No. 70065) using methanol/water and were detected by UV at 220nm and by evaporative light scattering using an Alltech 500 ELSD (Evaporative Light Scattering Detector.)
The UV detection did not resolve the individual glucopyranosides, while the ELSD produced distinct peaks for n-decyl B-D-glucopyranoside, n-octyl B-D-glucopyranoside and n-dodecyl B-D-glucopyranoside.
In ELSD, the mobile phase is first evaporated. Solid particles remaining from the sample are then carried in the form of a mist into a cell where they are detected by a laser.
Key Analyte(s)
n-decyl-beta-d-glucopyranoside
n-dodecyl beta-d-glucopyranoside
n-octyl beta-d-glucopyranoside
Equipment
Alltech Econosphere ColumnsAlltech Econosphere Columns are low-cost columns for common applications. They are available in 80Å pore size and are acid-washed for low metal content. They are available with eleven packing phases.
Technique
HPLCHigh Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.
Source
Alltech Associates, Inc.Alltech Associates, Inc., one of the leading suppliers of HPLC columns, manufactures and distributes HPLC, GC and IC columns, accessories and consumables and maintains a large searchable database of application notes and chromatograms on its website.
