Application Notes
Polystyrene MW Standards by HPLC/ELSD
Polystyrene molecular weight standards from 800 to 90,000 MW were separated by reversed phase HPLC and detected by evaporative light scattering.
Polystyrene MW Standards, Alltech Application Note 0009E, July 7, 2000.
Polystyrene molecular weight standards from 800 to 90,000 MW were separated isocratically in around 16 minutes by reversed phase HPLC on a Jordi Gel 500A, 500 x 10mm column (Part No. 100567) using 100% THF and were detected by evaporative light scattering using an Alltech 500 ELSD (Evaporative Light Scattering Detector.)
In ELSD, the mobile phase is first evaporated. Solid particles remaining from the sample are then carried in the form of a mist into a cell where they are detected by a laser.
Key Analyte(s)
polystyrene
Technique
HPLCHigh Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.
Source
Alltech Associates, Inc.Alltech Associates, Inc., one of the leading suppliers of HPLC columns, manufactures and distributes HPLC, GC and IC columns, accessories and consumables and maintains a large searchable database of application notes and chromatograms on its website.
