Application Notes
Egg Yolk Phospholipids by HPLC/ELSD
Egg yolk phospholipids were separated by reversed phase HPLC and detected by evaporative light scattering.
Egg Yolk Phospholipids, Alltech Application Note 0008E, May 2, 2000.
Egg yolk phospholipids were separated isocratically in around 20 minutes by reversed phase HPLC on a Nucleosil C18, 5µm, 250 x 4.6mm column (Part No. 89141) using chloroform/acetonitrile and were detected by evaporative light scattering using an Alltech 500 ELSD (Evaporative Light Scattering Detector.)
In ELSD, the mobile phase is first evaporated. Solid particles remaining from the sample are then carried in the form of a mist into a cell where they are detected by a laser.
Key Analyte(s)
phospholipids
Technique
HPLCHigh Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.
Source
Alltech Associates, Inc.Alltech Associates, Inc., one of the leading suppliers of HPLC columns, manufactures and distributes HPLC, GC and IC columns, accessories and consumables and maintains a large searchable database of application notes and chromatograms on its website.
