Amino Acids in Human Urodilatin Hydrolysate

Amino acids in human urodilatin hydrolysate (6N HCl/15 hours) were separated by gradient elution using a GROM-SIL FMOC-2 column and detected by fluorescence at 263 and 313nm.

Amino Acid Analysis of Human Urodilatin Hydrolysate (6N HCl/15 hours), Grom Application Number 10057

Amino acids in human urodilatin hydrolysate (6N HCl/15 hours) were separated using a GROM-SIL FMOC-2 column (250 x 4mm, Part No. GSFM20512S2504) by gradient elution with 50 mM sodium acetate and acetonitrile 80/20 and 50 mM sodium acetate/acetonitrile 20/80 at pH 4.2 in around 50 minutes and detected by fluorescence at 263 and 313nm. The amino acids and other compounds separated were arginine, serine, aspartic acid, glutamic acid, threonine, glycine, alanine, tyrosine, proline, FMOC-OH, ammonia, methionine, phenylalanine, isoleucine, leucine, histidine and lysine.