Amino Acids in Bovine Serum Albumin Hydrolysate

Amino acids in bovine serum albumin hydrolysate (6N HCl/15 hours) were separated by gradient elution using a GROM-SIL FMOC-2 column and detected by fluorescence at 263 and 313nm.

Amino Acid Analysis of Bovine Serum Albumin Hydrolysate (6N HCl/15 hours), Grom Application Number 10056

Amino acids in a bovine serum albumin hydrolysate (6N HCl/15 hours) were separated using a GROM-SIL FMOC-2 column (250 x 4mm, Part No. GSFM20512S2504) by gradient elution with 50 mM sodium acetate and acetonitrile 80/20 and 50 mM sodium acetate/acetonitrile 20/80 at pH 4.2 in around 50 minutes and detected by fluorescence at 263 and 313nm. The amino acids and other compounds separated were arginine, serine, aspartic acid, glutamic acid, threonine, glycine, alanine, tyrosine, proline, FMOC-OH, methionine, valine, phenylalanine, isoleucine, leucine, histidine and lysine.