Application Notes
Triglycerides in Lip Balm by HPLC/ELSD
Triglycerides in a commercial lip balm, Chapstick were separated by reversed phase HPLC and detected by evaporative light scattering.
Triglycerides in Chapstick, Alltech Application Note 0005E, April 24, 2000.
Triglycerides in a commercial lip balm, Chapstick, were separated in around 10 minutes by reversed phase HPLC on a Hypersil C18, 5µm, 250 x 4.6mm column (Part No. 9879) using gradient elution with acetone and ethanol. The compounds were detected by evaporative light scattering using an Alltech 500 ELSD (Evaporative Light Scattering Detector.)
In ELSD, the mobile phase is first evaporated. Solid particles remaining from the sample are then carried in the form of a mist into a cell where they are detected by a laser.
Key Analyte(s)
triglycerides
Technique
HPLCHigh Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.
Source
Alltech Associates, Inc.Alltech Associates, Inc., one of the leading suppliers of HPLC columns, manufactures and distributes HPLC, GC and IC columns, accessories and consumables and maintains a large searchable database of application notes and chromatograms on its website.
