Protein Hydrolysate Standard

Amino acids in a protein hydrolysate standard (6N HCl/15 hours) were separated by gradient elution using a GROM-SIL FMOC-2 column and detected by fluorescence at 263 and 313nm.

Protein Hydrolysate Standard (6N HCl/15 hours), Grom Application Number 10054

Amino acids in a protein hydrolysate standard (6N HCl/15 hours) were separated using a GROM-SIL FMOC-2 column (250 x 4mm, Part No. GSFM20512S2504) by gradient elution with 50 mM sodium acetate and acetonitrile 80/20 and 50 mM sodium acetate/acetonitrile 20/80 at pH 4.2 in around 50 minutes and detected by fluorescence at 263 and 313nm. The amino acids and other compounds separated were cysteine sulphinic acid, arginine, serine, aspartic acid, glutamic acid, threonine, glycine, alanine, tyrosine, proline, FMOC-OH, ammonia, methionine, valine, nor-valine, phenylalanine, isoleucine, leucine, histidine and lysine.