Application Notes
Carbohydrates - Sugar Standards by HPLC/ELSD
Six sugar standards were separated by reversed phase HPLC and detected by evaporative light scattering.
Carbohydrates – Sugar Standards by HPLC/ELSD, Alltech Application Note 0004E, October 23, 2000.
A mixture of six sugars were separated in around 26 minutes by reversed phase HPLC on an Adsorbosphere NH2, 250 x 4.6 mm column (Part No. 287242) using both isocratic and gradient elution with acetonitrile/water and detected by evaporative light scattering using an Alltech 500 ELSD (Evaporative Light Scattering Detector.)
Fructose, glucose, sucrose, maltose and lactose were detected by both forms of elution, while raffinose was detected using gradient only.
In ELSD, the mobile phase is first evaporated. Solid particles remaining from the sample are then carried in the form of a mist into a cell where they are detected by a laser.
Key Analyte(s)
fructose
glucose (dextrose)Glucose (dextrose)is a simple sugar used in foods and drinks. It is most often measured using HPLC and GC/MS.
lactoseLactose (milk sugar) is a disaccharide found in milk and used pharmaceutical tablets. It is most often measured using HPLC and GC/MS.
maltose
raffinose
sucrose
Equipment
Alltech Adsorbosphere ColumnsAlltech Adsorbosphere® Columns are designed using high temperature bonding for exceptional surface coverage and stability for common applications. They are available in pore sizes from 60-90Å and come in four varieties.
Technique
HPLCHigh Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.
Source
Alltech Associates, Inc.Alltech Associates, Inc., one of the leading suppliers of HPLC columns, manufactures and distributes HPLC, GC and IC columns, accessories and consumables and maintains a large searchable database of application notes and chromatograms on its website.
