Application Notes
Triglycerides in Olive Oil by HPLC/ELSD
Triglycerides in olive oil were separated by reversed phase HPLC and detected by evaporative light scattering.
Triglycerides – Olive Oil, Alltech Application Note 0003E, May 8, 2000.
Triglycerides in olive oil were separated by reversed phase HPLC on a Hypersil C18, 5µm, 250 x 4.6mm column (Part No. 9879) using gradient elution with acetonitrile and methanol.
UV detection at 225 nm showed almost no discernible peaks, while detection by evaporative light scattering detection (ELSD) using an Alltech 500 ELSD (Evaporative Light Scattering Detector) found more than a dozen individual components over a 40-minute period.
In ELSD, the mobile phase is first evaporated. Solid particles remaining from the sample are then carried in the form of a mist into a cell where they are detected by a laser.
Key Analyte(s)
Triton X 100
Technique
HPLCHigh Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.
Source
Alltech Associates, Inc.Alltech Associates, Inc., one of the leading suppliers of HPLC columns, manufactures and distributes HPLC, GC and IC columns, accessories and consumables and maintains a large searchable database of application notes and chromatograms on its website.
