Application Notes
Asparagine by HPLC/UV Using Chirobiotic T Column
The enantiomers of the amino acid asparagine were separated by reversed phase HPLC using a Chirobiotic T column and detected by UV at 210nm.
Asparagine, Astec Application Report 5002
Asparagine, CAS No. 70-47-3, an amino acid was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol, water (50:50). The enantiomers were detected by UV at 210nm.
Technique
HPLC/UVIn HPLC, ultraviolet (UV) detectors measure absorption of radiation from chromophores in eluted compounds over the range 190-400nm.
Source
Astec (Advanced Separation Technologies, Inc.)Astec (Advanced Separation Technologies) is a leader in the production and sale of chiral chromatography columns for LC and capillary GC.
