Application Notes
Cottonseed, Linseed Oil by HPLC/ELSD
Cottonseed and linseed oils separated by HPLC into components that are detected by evaporative light scattering.
Seed Oils - Cottonseed, Linseed, Alltech Application Note 0002E, May 21, 2000.
In separate determinations, cottonseed and linseed oil were separated by reversed phase HPLC on a Solvent Miser C18, 5µm, 250 x 2.1mm column (Part No. 9945) using gradient elution with acetonitrile and ethanol.
Their respective components were detected by evaporative light scattering detection (ELSD) using an Alltech 500 ELSD (Evaporative Light Scattering Detector.) In ELSD, the mobile phase is first evaporated. Solid particles remaining from the sample are then carried in the form of a mist into a cell where they are detected by a laser.
Several unidentified components in each oil eluted over a 40-minute period.
Key Analyte(s)
cottonseed oilCottonseed oil, derived from the seeds of the cotton plant, contains fatty acids and is used in soaps and margarine. It is most often measured using HPLC, GC/FID and GC/MS.
Technique
HPLCHigh Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.
Source
Alltech Associates, Inc.Alltech Associates, Inc., one of the leading suppliers of HPLC columns, manufactures and distributes HPLC, GC and IC columns, accessories and consumables and maintains a large searchable database of application notes and chromatograms on its website.
