Application Notes
Cholesterol by HPLC/ELSD
Cholesterol separated by HPLC and detected by evaporative light scattering.
Cholesterol, Alltech Application Note 0001E, May 8, 2000.
A reversed phase HPLC chromatogram of a 104ng/µl cholesterol standard is depicted. Cholesterol was separated in 16 min on a Solvent Miser C18, 5µm, 250 x 2.1mm column (Part No, 9945) using gradient elution with acetonitrile and methanol.
Cholesterol was detected by evaporative light scattering detection (ELSD) using an Alltech 500 ELSD (Evaporative Light Scattering Detector.) In ELSD, the mobile phase is first evaporated. Solid particles remaining from the sample are then carried in the form of a mist into a cell where they are detected by a laser.
Key Analyte(s)
cholesterolCholesrerol is a steroid produced naturally and obtained from animals in the diet. Its level in the blood is associated with cardiovascular disease. It is most often measured using HPLC, GC/FID and GC/MS.
Technique
HPLCHigh Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.
Source
Alltech Associates, Inc.Alltech Associates, Inc., one of the leading suppliers of HPLC columns, manufactures and distributes HPLC, GC and IC columns, accessories and consumables and maintains a large searchable database of application notes and chromatograms on its website.
