Application Notes
The enantiomers of the bronchodilator drug fenuterol were separated by reversed phase HPLC using a Chirobiotic V column and detected by UV at 254nm.
Fenuterol, Astec Application Report 118
Fenuterol, CAS No 13392-18-2 a bronchodilator drug was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using THF, ammonium nitrate 20mM (10:90), pH 5.5. The enantiomers were detected by UV at 254nm.
In HPLC, ultraviolet (UV) detectors measure absorption of radiation from chromophores in eluted compounds over the range 190-400nm.
Astec (Advanced Separation Technologies) is a leader in the production and sale of chiral chromatography columns for LC and capillary GC.