Application Notes
The enantiomers of 1,3-dicarboxylic acid allene, a chiral compound used in asymmetric synthesis were separated by reversed phase HPLC using a Chirobiotic T column and detected by UV at 254nm.
1,3-dicarboxylic acid allene, Astec Application Report 271
1,3-dicarboxylic acid allene, CAS No. NR, a chiral compound used in asymmetric synthesis was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using 10% water in methanol, acetic acid, TEA (100:0.5:0.5). The enantiomers were detected by UV at 254nm.
In HPLC, ultraviolet (UV) detectors measure absorption of radiation from chromophores in eluted compounds over the range 190-400nm.
Astec (Advanced Separation Technologies) is a leader in the production and sale of chiral chromatography columns for LC and capillary GC.