Dispersion in Chromatography Columns - The Van Deemter Equation > Page 51
and C=0.00250 sec.
If the mean particle size was 0.00085 cm, then as,
Then, 0.00117 = 2l 0.00085 or, l = 0.69
Giddings (11) determined theoretically that, for a well packed column (l) should take a value of about 0.5 and thus the column used was reasonably well packed.
In a similar manner, as B = 2gDm
Then, 0.0000175 = 2gDm
Katz et al (12) determined the diffusivity of hexamethylbenzene in 4.83%w/v ethyl acetate in n-decane and found it to be 1.17x10-5 cm2/sec,