The fraction (c) of the complexed solute is given as,
or
Substituting
for (1-c) in the equation for (
),
Unfortunately,
it is impossible to separate the terms (
) and 
and,
thus, the procedure must be complicated further by the introduction of four
additional specific retention volumes. Experimental values for these can be
used (with certain assumptions)
to obtain a
value for (K') and consequently a value for (
).
