Thus, Vo = Qto where Q is the flow rate in ml/min.
The retention time (tr) is the time elapsed between the injection point and the peak maximum. Each solute has a characteristic retention time.
The retention volume (Vr) is the volume of mobile phase passed through the column between the injection point and the peak maximum.
Thus, Vr = Qtr where Q is the flow rate in ml/min.
Each solute will also have a characteristic retention volume.
The corrected retention time (t'r) is the time elapsed between the dead point and the peak maximum.
The corrected retention volume (V'r) is the volume of mobile phase passed through the column between the dead point and the peak maximum. It will also be the retention volume minus the dead volume.
Thus, V'r = Vr - Vo = Q(tr - to) where Q is the flow rate in ml/min.
