The chosen
column was assumed to be 40 cm long, 2 cm I.D., having a dead volume of 75.4 ml
(
). The maximum sample volume was calculated for a range
of separation ratio values (a) and
capacity ratio values (k) for the first eluted peak. The results are shown in
figure 2.
Figure 2 Graph of Maximum Sample Volume against (k') for the First Solute
