Amazon Principles and Practices


Now,

The velocity of the band along the column (v) will be the ratio of the column length (L) to the retention time (tr)

Thus,

Now when then the solute will move through the column in the direction of the mobile phase and be eluted in the mobile phase at the end of the column.

 

And when then the solute will move through the column in the opposite direction to the mobile phase and be eluted by the moving stationary phase at the beginning of the column.


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