Table 2 Summary of the Physical and Chemical Properties of the Zorbax Column
Column Length 25 cm
Column Radius 2.3 mm
Column Packing Zorbax C8 Reverse Phase
Carbon Content of Reverse Phase 10.0%w/w
Equivalent Decane Content (Dimethyl Octane) 11.8%w/w
Total Column Volume 4.15 ml
Total Volume of Silica in the Column 0.96 ml
Total Volume of Stationary Phase in the Column 0.40 ml
Volume of Chromatographically Available Stationary Phase 0.49 ml
Volume of Chromatographically Unavailable Stationary Phase (by diff.) - total mobile phase in the column,
1. By weighing 2.79 ml
2. From the retention of the alkanes in n-octane extrapolated
to an alkane carbon number of 3 2.78 ml
3. From the volume fraction average of the isotopes retention
volume 2.77 ml
Total Interstitial Volume, value extrapolated from the retention
volumes of ions of different size 1.91 ml
Interstitial Moving Phase Volume
from the retention of 'silica smoke' 1.41 ml
Interstitial Static Phase volume, by difference 0.50 ml
Total Pore Volume. By Difference 0.87 ml
