Optimizing excitation and emission light wavelengths to obtain maximum sensitivity for a complex mixture can become quite involved as shown by the separation of some priority pollutants depicted in figure 40. The separation was carried out on a column 25 cm long, 4.6 mm in diameter and packed with a C18 reversed phase. The mobile phase was programmed from a 93% acetonitrile, 7% water to 99% acetonitrile, 1% water over a period of 30 minutes. The gradient was linear and the flow rate was 1.3 ml/min.
Courtesy of the Perkin Elmer Corporation
| 1 Naphthalene | 9 Chrysene |
| 2 Acenaphthene | 10 Benzo(b)fluoranthene |
| 3 Fluorene | 11 Benzo(k)fluoranthene |
| 4 Phenanthrene | 12 Benzo(a)pyrene |
| 5 Anthracene | 13 Dibenz (a,h)anthracene |
| 6 Fluoranthene | 14 Benzo(ghi)perylene |
| 7 Pyrene | 15 Indeno(123-cd)pyrene |
| 8 Benz(a)anthracene |
