Liquid Chromatography Detectors
by RPW Scott
part of the Chrom Ed. Series


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Figure 5 Elution Curves Presented as a Function of the Normalized Tube Length

 

It is seen that serious peak distortion can occur but as the length of the sensor cell is increased the distortion is reduced, but the dispersion increases. However, if the conduits to the cell are appropriately designed to produce secondary flow in the cell, then the parabolic velocity profile is destroyed, and the dispersion and peak distortion eliminated. The manner of entry of the mobile phase from the connecting conduits are, consequently, designed to produce this secondary flow and the manner in which this is achieved is shown in figure 6.


Related Topics

mobile phase peak elution
 
 
 

Journal of Chromatographic Science

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