Then 
(6)
and n = Tt(0), where (Tt(0)) will be the retention time of the solute under the defined pressure programming conditions.
Taking a
simple practical situation where the retention volume is 1000 ml on a given
column operating at a (g) value of 2
and the retention time of the solute is 10 minutes (600 seconds). The flow
properties of the column i.e. 
can be defined in the following manner,
From
equation (3) 
or 
. Thus, 
Substituting
for in (6),
(7)
Equation (7) can now be employed to calculate the change in retention time for a series of solutes separated under pressure programming conditions in the defined column
