Liquid Chromatography Detectors - The Fluorescence Detector > The Single Wavelength Excitation Fluorescence Detector > Page 58

An example of a separation monitored by a simple fluorescence detector is the separation of a mixture of priority pollutants shown in figure 37. The excitation light was approximately monochromatic at 254 nm and all wavelengths of the fluorescent light was sensed by the photo cell.


Column: 2 Pecosphere™–5C C18 (150 mm x 4.6 mm) in series. Mobile Phase: 90% acetonitrile/10% water. Flow rate: 2.0 ml/min. Detector Fluorescence (Excitation 254 nm total emission sensed). Sample: 20 ml of NBS Standard.


1. Naphthalene 2. Fluorene 3. Acenaphthene
4. Phenanthrene 5. Anthracene 6. Fluoranthracene
7. Pyrene 8. Benzo(a)anthracene 9. Chrysene
10. Benzo(b)fluoranthene 11. Benzo(k)fluoranthene 12. Benzo(k)fluoranthene
13. Dibenz(a,h)anthracene 14.Indeno(1,2,3,cd)pyrene 15. Benzo(ghi)perylene

Courtesy of the Perkin Elmer Corporation

Figure 37. Separation of the Priority Pollutants Monitored by the Simple Fluorescence Detector

There are some compromises between the expensive fluorescence spectrometer detector and the single wavelength excitation fluorescence detector. Some have a single monochromators that select the wavelength of the excitation light, others employ a single monochromator to select the emission wavelength or provide emission spectra.